BIOPHARMACEUTICS – PHARMACOKINETICS (LABORATORY WORK)

(Compulsory / Teaching hours per week: 9 / Assessment: laboratory exercises rating (40%), final written exam (60%) / Credits: 3)

Teachers:

Laboratory Tutorials:
G. Valsami (Associate Professor), M. Symillidou (Associate Professor), M. Vertzoni (Assistant Professor)
Laboratory Exercises:
C. Reppas (Professor), S. Markantonis (Professor), G. Valsami (Associate Professor), N. Drakoulis (Associate Professor), M. Symillidou (Associate Professor), M. Vertzoni (Assistant Professor), A. Dokoumetzidis (Assistant Professor), V. Karalis (Assistant Professor))

Course site (e-class):  https://eclass.uoa.gr/courses/PHARM199/  

Topics:

    • Aqueous solubility of drug substances: Determination of the aqueous solubility of dipyridamole. 
    • Aqueous solubility of drug substances: Effect of β-cyclodextrin on the aqueous solubility of dipyridamole.
    • In vitro dissolution of immediate release pharmaceutical products: Dissolution rate: Effect of pH on the dissolution rate of dipyridamole immediate release tablets, 
    • In vitro dissolution of immediate release pharmaceutical products: Comparison of dissolution curves using the similarity factor, f2.
    • Protein binding of drugs:  Study of the to the interaction of Cu++ ions with Bovine Serum Albumin (BSA).
    • Protein binding of drugs: Application of Scatchard model for the determination of the binding parameters for the interaction of Cu++ with BSA. 
    • Analysis of in vitro and in vivo data: Calculation of basic pharmacokinetic parameters from in vivo data after per os administration. Evaluation of dissolution kinetics based on in vitro dissolution data.
    • Analysis of in vitro and in vivo data: In vitro – in vivo correlation for per os administered drugs (Level A, B and C correlations).