(Compulsory / Teaching hours per week: 4 / Laboratory hours per week: - /Assessment: final exam at the end of each term or September/ Credits: 5 )


Irene Panderi, Professor
Yannis Dotsikas, Assistant Professor 

Course site (e-class):


    • Oxidometric methods: Bromimetric assays, determinations with KBrO3, assays with Ce(SO4)2, with TiCl3, with NaNO2 and with limited application reagents.
    • Complexpmetric methods: General principles of EDTA titrations. Selective titrations in multimetallic systems - binding and releasing agents. 
    • Precipitation titrations: determination of ionized and organically bound halogen. Examples of the application of the above methods in Pharmaceutical Analysis - reference to the respective Pharmacopoeia monographs - comparison with other methods of determining the same active compound.
    • Experimental Design. Introduction, screening designs, optimization designs, surface response methodology, desirability function.
    • UV / Vis spectrophotometry: General principles of technique, organology, instrument calibration. UV / Vis spectra of representative pharmaceutical molecules. Use of the technique in the determination of pKa values ​​and in quantitative pharmaceutical analysis. Differential spectrophotometry. Derivative spectrophotometry. Applications of UV / Vis absorption spectrophotometry to raw material and pharmaceutical products with examples of applications from the Pharmacopoeia. Spectrophotometric determinations after modifying the structure of the specified product. Colorimetric determinations.
    • High performance liquid chromatography (HPLC): General principles of technique, organology, stationary and mobile phases, retention mechanisms. Applications of HPLC to quantitative determinations of drugs in formulations with examples from the pharmacopoeia. Determinations by specialized HPLC techniques: ion-pair chromatography, ion-exchange chromatography, chiral chromatography. Mass chromatography-mass spectrometry (LC-MS): General principles of the technique, applications of the technique to the quality control of related compounds.